Food Sci. Barnes and Thornley (1966) J. A bacteriological growth medium selective for Pseudomonas fluorescens, said selective medium comprising a nutrient component capable of supporting growth of P. fluorescens and a compound of the formula ##STR3## where R is a halogen and R. 2. Pseudomonas putida and P. fluorescens were distributed widely but unevenly through the soils and plant roots.P. Russell et al. Overall, P. fluorescens was the bacterium that was selectively enumerated on Day 0 carcasses. Three replicate trials were conducted (Table 2). Pseudomonas fluorescens was one of seven species selectively enumerated using BHI-I. using Test Code 6. Pooni and Mead (1984) Food Microbiol. 48:1179-1183; Randolph et al. Rev. Cox et al. Generally, the by-products of glucose metabolism do not substantially contribute to spoilage defects in proteinaceous foods; however, as glucose is depleted, spoilage bacteria utilize other substrates, such as amino acids, that are present in skin and muscle. J. Poultry Sci. For plates on which bacterial species other than P. fluorescens were isolated, that plate was examined to determine the bacterium that was overwhelmingly predominant on that plate and thus, would be the organism that produced the impedance change detected by the instrument.
Milk Food Technol. Questions? Only chicken was evaluated in this study; however, because chicken has the most diverse microflora of the animal derived foods, this method can be used to predict the potential shelf-life of other fresh foods of animal origin besides chicken, including other poultry including, without limitation, duck, goose, dove, turkey, pheasant and quail; meats including, but not limited to, beef, veal, pork, lamb, beefalo, buffalo, kangaroo, ostrich and alligators; fresh water and salt water shellfish, and creams and milks of various origins. Only chicken was evaluated in this study; however, because chicken has the most diverse microflora of the animal derived foods, this method can be used to predict the potential shelf-life of other fresh foods of animal origin besides chicken, including other poultry including, without limitation, duck, goose, dove, turkey, pheasant and quail; meats including, but not limited to, beef, veal, pork, lamb, beefalo, buffalo, kangaroo, ostrich and alligators; fresh water and salt water shellfish, and creams and milks of various origins. Four selective media for Pseudomonas strains producing fluorescent pigment (P-l medium), Pseudomonas putida strains (P-2 medum) and Pseudomonas fluorescens strains (P-3 and P-4 media) were proposed on the basis of the assimilation of carbon sources by the strains. We tested the selectivity and sensitivity of M9-PP-agar by measuring the growth of 68 bacterial strains from 36 different species on this medium. [PMC free article] Sands DC, Rovira AD. One hundred and three strains of Pseudomonas species producing fluorescent pigment were isolated from soils and plant roots, and used for the evaluation of these selective media. Poultry Sci. Results are reported as % transmittance. When analyzing broiler carcass rinses using this medium, detection times were erratic and the instrument was unable to interpret the capacitance curves consistently. Day 6 Rep 1 Rep 2 Rep 3 Bacterium Bird 1 Bird 2 Bird 1 Bird 2 Bird 1 Bird 2, Number of isolates recovered from broiler chicken carcasses obtained from the chiller exit of a commercial processing plant and analyzed after 9 days of storage at 3° C. using brain heart infusion broth with Pseudomonas fluorescens selective additive (BHI-PSA) as the culture medium. LPDT determinations are an excellent means of estimating the total number of psychrotrophs and P. fluorescens on fresh broiler chicken carcasses and other fresh foods of animal origin. PPC correlated well to day of storage and odor production, indicating that it is acceptable for predicting the potential shelf-life of fresh chicken. All stock solutions containing antibiotics, Irgasan, and TMAO were filter sterilized using 0.2Î¼ filters (SuporÂ® AcrodiscÂ® 25 syringe filter, Gelman Sciences, Ann Arbor, Mich.). 35:574-576; Janzen et al. https://doi.org/10.1016/j.mimet.2020.105911. 156-157!, have also been used as a general indicator of potential shelf-life; however, enumeration of psychrotrophic spoilage bacteria using this technique is difficult, cumbersome, and time consuming. %PDF-1.4 What is needed is a means to predict the potential shelf-life of fresh foods of animal origin, preferably by rapidly enumerating the populations of bacteria that are primarily responsible for producing spoilage defects in these products immediately after processing. Easter et al.
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