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selective media for pseudomonas fluorescens


Food Sci. Barnes and Thornley (1966) J. A bacteriological growth medium selective for Pseudomonas fluorescens, said selective medium comprising a nutrient component capable of supporting growth of P. fluorescens and a compound of the formula ##STR3## where R is a halogen and R. 2. Pseudomonas putida and P. fluorescens were distributed widely but unevenly through the soils and plant roots.P. Russell et al. Overall, P. fluorescens was the bacterium that was selectively enumerated on Day 0 carcasses. Three replicate trials were conducted (Table 2). Pseudomonas fluorescens was one of seven species selectively enumerated using BHI-I. using Test Code 6. Pooni and Mead (1984) Food Microbiol. 48:1179-1183; Randolph et al. Rev. Cox et al. Generally, the by-products of glucose metabolism do not substantially contribute to spoilage defects in proteinaceous foods; however, as glucose is depleted, spoilage bacteria utilize other substrates, such as amino acids, that are present in skin and muscle. J. Poultry Sci. For plates on which bacterial species other than P. fluorescens were isolated, that plate was examined to determine the bacterium that was overwhelmingly predominant on that plate and thus, would be the organism that produced the impedance change detected by the instrument.

Seven of the eight media analyzed allowed P. fluorescens to multiply while suppressing the growth of the other bacterial isolates. Results are reported as % transmittance. Therefore, reduction of carbenicillin is not possible when selecting for growth of P. fluorescens. General purpose medium plus (GPMP, bioMerieux Vitek, Inc., Hazelwood, Mo.) One milliliter of rinse fluid from each carcass was placed into 9 mL of BHI-PSA and incubated until a detectable signal occurred in the capacitance measurement. ); Potassium sulfate, 10 g (Sigma Chemical Co.) Irgasan, 10,000 μg (Irgasan DP300, CAS#33-80345, Ciba-Geigy, Greensboro, N.C.). Because media were different wit regard to baseline transmittance, + and - symbols are also reported: +, bacteria produced visible turbidity in medium, ±, bacteria produced slight turbidity, -, bacteria produced no visible turbidity. & Terms of Use. Brit.) Wolin et al. Viehweg et al. 12:197-203 reported that Pseudomonas fluorescens constituted approximately 80% of the bacterial species on meat in the latter part of storage. 12:197-203 reported that Pseudomonas fluorescens constituted approximately 80% of the bacterial species on meat in the latter part of storage. 53:357-365. In a preferred embodiment, selective growth of P. fluorescens is effected by the combination of Irgasan, carbenicillin and nitrofurantoin in a bacteriological medium … Research Food Invest. ); NaCl, 20 g (J.T. Board (Gr.Brit) Special Report No. The carcasses were sampled on the day of collection by rinsing in 100 mL of sterile deionized water according to the procedure described in Cox et al. > 47:471-475; Fitz-Gerald and Deeth (1983) Austral. UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC., G, Free format text: Total psychrotrophic plate counts (PPC)±standard error of the mean (SEM) for broiler chicken carcasses analyzed immediately (Day 0), or sampled after holding at 3° C. for 3, 6, 9 or 12 d are presented in Table 17. As soon as capacitance detection times (DT) were recorded, 1 mL of the sample was immediately taken from the module well, placed into 9 mL of sterile Bacto-peptone (Difco Laboratories, Detroit, Mich.), diluted to 10-6, 10-7, or 10-8, spread onto duplicate plate count agar (PCA, Difco Laboratories, Detroit, Mich.) plates, and incubated at 25° C. for 48 h according to the procedure described by Russell et al. Mead and Adams (1977) Br. 4 Control = baseline colorimetric values for the medium without inoculum. (1957) Food Technol. Data for growth of bacteria in BHI with various levels of cephalothin, carbenicillin, NaCl, and trimethylene N-oxide (TMAO) are presented in Table 5. (1981) Poultry Sci. For media containing NaCl, NaCl was added directly to BHI before sterilization. BHI containing nitrofurantoin (4 μg), carbenicillin (120 μg), and Irgasan (25 μg) per mL was excellent for enumeration of P. fluorescens from broiler chicken carcass rinses using capacitance microbiology at 25° C. The average times required to enumerate P. fluorescens Day 0, 3, 6, 9, 12 and 15 samples were 22.4, 17.2, 12.0, 4.6, 2.9 and <1.0 h respectively. 2 Media 16 consisted of BHI broth with the following additives per mL: 1) carbenicillin (200 μg); 2) carbenicillin (400 μg); 3) carbenicillin (600 μg); 4) cephalothin (500 μg), carbenicillin (300 μg), NaCl (10 mg), TMAO (1 mg); 5) cephalothin (1000 μg), carbenicillin (300 μg), NaCl (10 mg), TMAO (1 mg); 6) cephalothin (500 μg), carbenicillin (600 μg), NaCl (10 mg), TMAO (1 mg). However, PFPC estimations required 48 hours to conduct, thereby limiting its use as a practical method. We, therefore, developed a highly specific process for the routine isolation of new P. protegens strains from the soil environment, based on the use of a semi-selective medium and the specific color of colonies. Additives Used to Select for the Growth of Pseudomonas fluorescens. 9. 60:768-770. None of these media permitted P. fluorescens to multiply rapidly enough to be used in a capacitance assay. Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. Selective media for Pseudomonas, Pricing & availability is not currently available. 2 Pseudomonas isolation medium without agar (PIMAGAR) consisted of the following ingredients per liter of deionized water: Bactopeptone (20 g), Magnesium chloride (1.4 g), Potassium sulfate (10 g), and Irgasan (10 mg). 45, London: H.M.S.O. Also provided is a fast, reliable and economical method for the assessment of microbial quality of a fresh animal product, such as poultry, beef, fish, shellfish, milk or the like, using media selective for the growth of P. fluorescens and detection methods for microbial growth in the selective media. Overall, LPDT estimations only required 1 to 26 h to conduct. The present inventin provides methods and compositions for the specific detection of Pseudomonas fluorescens and for the selective growth of this bacterium. 15:765-770; Wolin et al. (1992) in: Compendium of Methods for the Microbiological Examination of Foods.

Milk Food Technol. Questions? Only chicken was evaluated in this study; however, because chicken has the most diverse microflora of the animal derived foods, this method can be used to predict the potential shelf-life of other fresh foods of animal origin besides chicken, including other poultry including, without limitation, duck, goose, dove, turkey, pheasant and quail; meats including, but not limited to, beef, veal, pork, lamb, beefalo, buffalo, kangaroo, ostrich and alligators; fresh water and salt water shellfish, and creams and milks of various origins. Only chicken was evaluated in this study; however, because chicken has the most diverse microflora of the animal derived foods, this method can be used to predict the potential shelf-life of other fresh foods of animal origin besides chicken, including other poultry including, without limitation, duck, goose, dove, turkey, pheasant and quail; meats including, but not limited to, beef, veal, pork, lamb, beefalo, buffalo, kangaroo, ostrich and alligators; fresh water and salt water shellfish, and creams and milks of various origins. Four selective media for Pseudomonas strains producing fluorescent pigment (P-l medium), Pseudomonas putida strains (P-2 medum) and Pseudomonas fluorescens strains (P-3 and P-4 media) were proposed on the basis of the assimilation of carbon sources by the strains. We tested the selectivity and sensitivity of M9-PP-agar by measuring the growth of 68 bacterial strains from 36 different species on this medium. [PMC free article] Sands DC, Rovira AD. One hundred and three strains of Pseudomonas species producing fluorescent pigment were isolated from soils and plant roots, and used for the evaluation of these selective media. Poultry Sci. Results are reported as % transmittance. When analyzing broiler carcass rinses using this medium, detection times were erratic and the instrument was unable to interpret the capacitance curves consistently. Day 6 Rep 1 Rep 2 Rep 3 Bacterium Bird 1 Bird 2 Bird 1 Bird 2 Bird 1 Bird 2, Number of isolates recovered from broiler chicken carcasses obtained from the chiller exit of a commercial processing plant and analyzed after 9 days of storage at 3° C. using brain heart infusion broth with Pseudomonas fluorescens selective additive (BHI-PSA) as the culture medium. LPDT determinations are an excellent means of estimating the total number of psychrotrophs and P. fluorescens on fresh broiler chicken carcasses and other fresh foods of animal origin. PPC correlated well to day of storage and odor production, indicating that it is acceptable for predicting the potential shelf-life of fresh chicken. All stock solutions containing antibiotics, Irgasan, and TMAO were filter sterilized using 0.2μ filters (Supor® Acrodisc® 25 syringe filter, Gelman Sciences, Ann Arbor, Mich.). 35:574-576; Janzen et al. https://doi.org/10.1016/j.mimet.2020.105911. 156-157!, have also been used as a general indicator of potential shelf-life; however, enumeration of psychrotrophic spoilage bacteria using this technique is difficult, cumbersome, and time consuming. %PDF-1.4 What is needed is a means to predict the potential shelf-life of fresh foods of animal origin, preferably by rapidly enumerating the populations of bacteria that are primarily responsible for producing spoilage defects in these products immediately after processing. Easter et al.

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